hto.anno {iCellR} | R Documentation |
Demultiplexing HTOs
hto.anno(hto.data = "data.frame", cov.thr = 10, assignment.thr = 80)
hto.data |
HTO raw data |
cov.thr |
A number which avrage coverage is devided by to set a thershold for low coverage, default = 10. |
assignment.thr |
A percent above which you decide to set as a good sample assignment/HTO, default = 80. |
An object of class iCellR
my.hto <- read.table(file = system.file('extdata', 'dense_umis.tsv', package = 'iCellR'), as.is = TRUE) head(my.hto)[1:5] htos <- hto.anno(hto.data = my.hto) head(htos) boxplot(htos$percent.match)