read_redf {dpcR} | R Documentation |
Read digital PCR raw data
Description
Reads REDF (Raw Exchange Digital PCR format) data.
Usage
read_redf(input, ext = NULL)
Arguments
input |
name of the input file ( |
ext |
extension of the file (). |
Details
REDF (Raw Exchange Digital PCR format) data is preferably a .csv file with following columns:
- experiment
names of experiments
- replicate
indices of replicates
- assay
names of assays
- k
number of positive partitions
- n
total number of partitions
- v
volume of partition (nL)
- uv
uncertainty of partition's volume (nL)
- threshold
partitions with
k
equal or higher than threshold are treated as positve.- panel_id
indices of panels
Column panel_id
should be specified only in case of
array-based dPCR.
Value
An object of adpcr
or dpcr
type,
depends on the value of adpcr
parameter.
Author(s)
Michal Burdukiewcz, Stefan Roediger
[Package dpcR version 0.6 Index]