ddpcRquant {dpcR} | R Documentation |
Quantify droplets
Description
Cluster raw data from QX100 and QX200 systems.
Usage
ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
threshold.manual = NULL)
Arguments
path |
|
threshold.int |
|
reps |
|
blocks |
|
threshold.manual |
if |
Value
dpcr
object.
Note
This function is a modification of code was implemented using the code found on https://ddpcrquant.ugent.be/.
Author(s)
Wim Trypsteen, Matthijs Vynck, Jan De Neve, Pawel Bonczkowski, Maja Kiselinova, Eva Malatinkova, Karen Vervisch, Olivier Thas, Linos Vandekerckhove, Ward De Spiegelaere.
References
Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.